Journal: Brain Sciences

Article Title: Akkermansia muciniphila Is Beneficial to a Mouse Model of Parkinson’s Disease, via Alleviated Neuroinflammation and Promoted Neurogenesis, with Involvement of SCFAs

doi: 10.3390/brainsci14030238

Figure Lengend Snippet: Akk promoted neurogenesis in the hippocampus of PD mice, possibly through activating the BDNF/TrkB signaling pathway. ( A ) Representative IF images of DCX-positive cells (immature neuron marker). Scale bar = 200 μm. The high magnification of images is shown in the white box on the right. The scale bar is 50 μm. ( B ) Quantitative analysis of DCX-positive cells in the hippocampal DG region at 10× magnification ( n = 3). ( C ) Representative IF images of SOX2-positive cells (neural stem cell marker). Scale bar = 200 μm. The high magnification of images is shown in the white box area on the right. The scale bar is 50 μm. ( D ) Quantitative analysis of SOX2-positive cells in the hippocampal DG region at 10× magnification ( n = 3–4). ( E , F ) The representative WB images of BDNF and TrkB expression in the hippocampus. ( G , H ) WB analysis of BDNF/GAPDH ratio and TrkB/GAPDH ratio in hippocampus ( n = 6). Statistical comparison was conducted with one-way ANOVA followed by post hoc comparisons of LSD. * p < 0.05, ** p < 0.01.

Article Snippet: The details of the primary antibodies are as follows: rabbit anti-tyrosine hydroxylase (TH, 1:1000, AB152, Merck, Kenilworth, NJ, USA), rabbit anti-doublecortin (DCX, 1:1000, 13925, Proteintech, Wuhan, China), rabbit anti-sex determining region Y-box 2 (SOX2, 1:900, 97959, Abcam, Cambridge, UK), mouse anti-glial fibrillary acidic protein (GFAP, 1:300, sc-33673, Santa Cruz Biotechnology, Paso Robles, CA, USA) and mouse anti-ionized calcium binding adaptor molecule 1 (Iba-1, 1:300, GB12105, Servicebio, Wuhan, China).

Techniques: Marker, Expressing, Comparison